ВЛИЯНИЕ АНАЭРОБНЫХ УСЛОВИЙ НА ПРОДУКТИВНОСТЬ MEDUSOMYCES GISEVII

Abstract

The effect of limited anaerobic conditions on the productivity of Medusomyces gisevii was studied. An artificial nutrient medium with a volume of 0.4 litres, containing 25% of the inoculate, was used throughout the experiments. Sugar (100 g / L) acting as a carbohydrate, as well as extracts of black or green tea (2 g / L) or coffee (2 g / L) were added to the microorganism cultivation medium. The metabolic activity of the symbiont microorganisms was evaluated by acidification of the nutrient medium, which manifested itself in low pH values and increased electrical conductivity values. Anabiosis of the symbiont was created by layering the vegetable oil on the surface of the nutrient medium. Under the conditions of anabiosis, the absence (for the extracts of black and green teas) and limited growth (for coffee extract) of biocellulose were observed. Moreover, in all samples of Medusomyces gisevii symbionts, the activity of microorganisms decreased sharply under conditions of anabiosis; this was manifested in a decrease in pH values during the month of cultivation by only 1.6-7.3%, and electrical conductivity values by 2.9-41.5%. The obtained data indicate that both oxygen and biocellulose localised on the surface of the culture liquid are needed for normal functioning of Medusomyces gisevii.