СОРТ ВИНОГРАДА ЗОЛОТИНКА В КОЛЛЕКЦИИ IN VITRO

Abstract

The article devoted to the current topic of biodiversity conservation by modern bio-technology methods. The preservation of in vitro vine germ plasma is an alternative to the field collection, and the development of effective in vitro storage can save valuable biodiversity. The study includes a table vari-ety of grapes Zolotinka. The variety is of very early or early maturation with a pleas-ant muscat aroma of berries. The vineyards of this variety have a high degree of viral and phytoplasna infection. Before the preservation, the infection was rehabilitated by an apicial meristem (size 0.1–0.2 mm) and with the help of antibiotic Cefotaxim. To slow down growth processes, the growth regulator Emystym and the antibiotic Gen-tamicin were studied to create a slow-growing collection. It was found that the drug Emistim has an impact on growth pro-cesses After 140 days of cultivation, the greatest preservation of plants was noted in the variant with Emistim in concentration 10-12. This variant shows the lowest length of risogenic zone in the experiment and the greatest growth of plants. Inhibition of growth processes (risogen area length, plant growth, number of leaves) was found at a concentration of 10-8. Decrease of survival of microcuttings and the intensity of growth processes was found when the nutrient me-dium was supplemented by antibiotic Gen-tamicin in concentrations of 0.03–0.05 ml/l. There is also a higher viabil-ity of plants in variants with Gentamicin, especially at a concentration of 0.03 ml/l. Due to antibiotic, the duration of non-stop storage can be increased up to 7–10 months.