상업용 효소를 이용한 인삼잎 유래 ginsenoside로부터 Compound K 생산

Abstract

This study aimed to prepare a valuable minor ginsenoside Compound K, using ginsenosides from ginseng leaf, by-products of ginseng, with a food-grade commercial enzyme Cytolase PCL 5. The total content of major ginsenosides in ginseng leaf extract was 146.9 mg/g and that of the PPD-type ginsenosides such as Rb1, Rb2, Rc, Rd and F2 was 5.7, 11.5, 19.8, 16.4, and 9.8 mg/g, respectively. Cytolase PCL5 showed very efficient enzymatic activity to produce Compound K from the PPD-type gisenosides of ginseng leaf extract. The Cytolase PCL5 exhibited the highest activity at pH 4.5 and 65℃, and the half-lives of the enzyme at 50, 55, 60, 65, 70, and 75℃ were 118, 94, 87, 60, 47, and 8 h, respectively. During Compound K production, the optimal concentrations of PPD-type ginsenosides and enzyme were 10 mg/g and 100 U/ml, respectively. Under optimum conditions, Cytolase PCL5 produced Compound K from PPD-type ginsenosides with a molar yield of over 97% and productivity of 1 mmole/L/h within 10 h. These results suggest that the ginseng leaf is a potential producer of Compound K and Cytolase PCL5 is a very useful enzyme for Compound K production.