Abstract
The author did experimental zymohystochemical studies on the developmental stages (from immediately after birth to 180 days) of rabbit's orbicularis oris muscles to determine the optimal time for cleft lip and palate operation.The results were as follows:1. The activity of lactate dehydrogenase was high for 30 days after birth, but it had no differential activities for the developmental stages as the marker.2. Orbicularis oris muscle fibers showed both dark and light glyceraldehydc-3-phosphate dehydrogenase staining for 60 days after birth.3. When lip plasty was done after differentiation of orbicularis oris muscle, it seems that the appropriate stage for the lip plasty is later than 60 days after birth in rabbits, and later than one year and 4 months after birth in humans.
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