황련(黃連) 추출물의 항염효능에 관한 연구

Abstract

Objectives : This research has been done to investigate the anti-inflammatory effect of Coptidis Rhizoma extracts. Method : Coptidis Rhizoma was extracted by <TEX>$100^{\circ}C$</TEX> water. The extract (CC : Extract of Coptis chinensis rhizome) was used to examine its effects on the cell viability of mouse macrophage Raw 264.7 cell line. Also the production of nitric oxide (NO), the c-jun N-terminalkinase (JNK) activation and the production of cytokines such as (IL)-5 were evaluated in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. After the CC and LPS were applied to Raw 264.7 cells which were cultured for 24 hours, the MTT assay was performed. Result : The CC extracts didn't affect the viability of macrophage cells. However, the extracts inhibited the NO production and the JNK activation significantly in LPS-stimulated macrophage cells treated with 100 and <TEX>$200{\mu}g/mL$</TEX> concentrations. The CC extract, also, impeded the production of inflammation-related factors and cytokines such as KC, VEGF, MCP-1, GM-CSF, IL-<TEX>$1{\alpha}$</TEX>, IL-5, IL-6, and IL-12p40 in LPS-stimulated macrophage cells at the concentration higher than <TEX>$25{\mu}g/mL$</TEX>. The production of basic-FGF concentration of 50 and <TEX>$100{\mu}g/mL$</TEX>, the production of IP-10 at <TEX>$100{\mu}g/mL$</TEX>, and the production of IFN-<TEX>${\gamma}$</TEX> at <TEX>$25{\mu}g/mL$</TEX>, respectively. Conclusion : The CC prepared using <TEX>$100^{\circ}C$</TEX> water showed the significant anti-inflammatory effect such as the inhibition not only on the production of NO, KC, VEGF, MCP-1, GM-CSF, IL-<TEX>$1{\alpha}$</TEX>, IL-5, IL-6, and IL-12p40 in LPS-stimulated macrophage cells at or higher than the concentration of <TEX>$25{\mu}g/mL$</TEX>, but also on the JNK activation at 100 and <TEX>$200{\mu}g/mL$</TEX>.