Abstract
The technique for modeling the «time deficit» emotional stress (TDS) was proposed in the experiment on 40 mature male white outbred rats (weight 220-240 g), its stressing effect was compared to that of the «free swimming in a cage» model. To achieve the TDS, individual rats were placed in a spiral-shaped tube that was quickly filled with water, which forced the rat to quickly move upwards. The classical signs of stress were observed — increased adrenal gland relative weight (31%), decreased thymus and spleen weight (26 and 14%), damaged stomach lining (in 80% of animals: 1st grade in 20% of rats, 2nd or 3rd grade in the other 60%, in 1:1 ratio; hemorrhage abundance: 2 hemorrhages for animal in 30%, 3 in 40%, 4 in 10% of rats, damage index: 4.7), altered serum corticosteroids (increased by 43%) and insulin (decreased by 19%) concentrations, activation of thyroid function (increased total and free triiodothyronine and thyroxine concentrationsin the blood by 18-32%). The horizontal locomotion and physical endurance were increased in animals following the TDS, whereas their vertical locomotion decreased as they developed anxiety. In comparison with the existing models, the proposed technique of the emotional stress modeling allows for the individual testing of animals, provides total removal of their waste and does not involve the physical trauma, which allows for the more objective studies of the mechanisms of stress injure development.
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