파종 방법에 따른 고려인삼의 대사체 비교

Abstract

*Department of Medicinal Crop Research, NIHHS, Rural Development Administration, Eumseong 369-873, Korea.**Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.***College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.****Department of Food Science and Technology, Oregon State University, Corvallis, OR 97330, USA.ABSTRACT : Ginsenosides of roots in Panax ginseng were analyzed by metabolic-targeting HPLC using the partial leastsquares discriminant analysis (PLS-DA) and compared depending on sowing methods between direct seeding andtransplanting method. Score plots derived from PLS-DA could identify the sowing method between the direct seeding andtransplanting method in P. ginseng roots. The ginsenoside compounds were assigned as Rg1, Re, Rf, Rg2, Rb1, Rc,Rb2, Rb3, and Rd. Contents of Re, Rf, Rg2, Rb1, Rc, Rb3, and Rd of main roots produced from the transplanting methodwere relatively higher than those of samples produced from direct seeding method. Also, contents of Rg1, Re, Rf, Rg2, Rb1,Rc, Rb2, Rb3, and Rd of lateral roots from the transplanted samples were relatively higher than those of samples producedfrom direct seeding method. Therefore, HPLC with PLS-DA analysis can be a straightforward tool for identificationof ginsenosides in main or lateral roots of P. ginseng obtained from two different seeding methods between directand transplanting methods.Key Words : Panax ginseng Root, Sowing Method, Metabolic Targeting, HPLC