Атомно-силовая микроскопия эритроцитов больных панкреонекрозом

Abstract

Th e purpose of the study. Improve the results of early diagnosis of pancreonecrosis based on the examination of the cytoplasmic membrane of red blood cells. Material and methods. The morphological and physiological state of erythrocyte was examined using an Integra Aura atomic power microscope (CJSC NTMDT, Russia). A total of 37 people without somatic pathology and 38 patients with pancreonecrosis were examined. Th e cell membrane of each erythrocyte was scanned as well as each cell as a whole. A total of 940 red blood cells were examined. The Nova soft ware was used to process the data received with the AFM. Results. Our study has shown that the shape of the erythrocyte does not change in pancreonecrosis. However, structural changes of the membrane occur manifested by formation of multiple erosions on its surface against the background of POL activation, which leads to its deformation and increased adhesion. These changes reduce the transport capacity of the blood, which, in our opinion, leads to an increase in the general intoxication of the body and to deterioration in the condition of the patient. Conclusion. Apparently, the accumulation of lipid peroxidation products in the membrane causes its reorganisation, the number of sedentary conglomerates and lipid clusters increases, leading to a decrease in the speed of movement of protein molecules against the background of the rigidity of the erythrocyte membrane and deformation of its surface. In addition, increased adhesion of the erythrocyte membrane seems to disrupt not only the gas transmission function of the erythrocyte, but also the microrheological status of the blood, binding and transfer of amino acids, lipids, viruses and drugs.