콘크리트 구조물에서 분리한 미생물의 다양성 평가

Abstract

The microorganisms isolated directly from concrete structures were identified by 16S ribosomal RNA analysis, and a total of 75 microorganisms were finally identified. The DNA sequence information of the 75 microorganisms were registered to GenBank of NCBI (National Center for Biotechnology Information), USA, by the name of each microorganism to make the final report as the microorganisms isolated from concrete structures. These 75 microorganisms were identified and largely classified into three kinds of bacteria. There were 33 High G+C Gram positive Actinobacteria (44.00%), 23 Low G+C Gram positive Bacilli (30.67%), and 19 Gram negative Proteobacteria (25.33%), showing relatively diversely distribution on the concrete structures. The Nucleotide Blast Search of the 75 microorganisms indicated that they consisted of 29 Genus and 49 Species. Actinobacteria were finally identified to be the most prevalent on the concrete structures with relatively diverse species in this study. A variety of effective microorganisms could be assorted from these diverse microorganisms on the concrete structures, and those groups of strains, which are candidates for new species through various physiological and biochemical experiments, are expected to be very important research data from the perspective of environmentally friendly concrete.