Abstract
This study was performed to evaluate the antioxidant and anti-inflammatory of methanol extract from the jujube seed. Cell viability and production of nitric oxide (NO) was determined by MTT assay and Griess assay. Cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and mitogen-activated protein kinase (MAPKs) expression were detected by western blot analysis. The production of prostaglandin E₂ (PGE₂) and tumor necrosis factor alpha (TNF-α) were determined by enzyme-linked immunosorbent assay. The antioxidant effects of jujube seed were measured based on polyphenol and flavonoid contents. Jujube seed extracts decreased the production of NO, PGE₂ and TNF-α in a dose-dependent manner, and also reduced the expression of iNOS, COX-2 protein. Additionally, jujube seed extracts reduced the expression levels of inhibitor of kappa B (IkB), nuclear factor kappa B (NF-kB) and MAPKs protein. Jujube seed extracts were found to have 180.56 ㎍/㎎ and 36.67 ㎍/㎎ high polyphenol and flavonoid contents. These results provide evidence for the antioxidant and anti-inflammatory effects of jujube seed. Products made of jujube seed will be extracted and it will be confirmed to be functional materials in the future.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have