Abstract
This study was performed to evaluate the antioxidant and anti-inflammatory of methanol extract from the jujube seed. Cell viability and production of nitric oxide (NO) was determined by MTT assay and Griess assay. Cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and mitogen-activated protein kinase (MAPKs) expression were detected by western blot analysis. The production of prostaglandin E₂ (PGE₂) and tumor necrosis factor alpha (TNF-α) were determined by enzyme-linked immunosorbent assay. The antioxidant effects of jujube seed were measured based on polyphenol and flavonoid contents. Jujube seed extracts decreased the production of NO, PGE₂ and TNF-α in a dose-dependent manner, and also reduced the expression of iNOS, COX-2 protein. Additionally, jujube seed extracts reduced the expression levels of inhibitor of kappa B (IkB), nuclear factor kappa B (NF-kB) and MAPKs protein. Jujube seed extracts were found to have 180.56 ㎍/㎎ and 36.67 ㎍/㎎ high polyphenol and flavonoid contents. These results provide evidence for the antioxidant and anti-inflammatory effects of jujube seed. Products made of jujube seed will be extracted and it will be confirmed to be functional materials in the future.
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