Abstract
The article is related to the investigation of adsorption potential of proteins ( albumin, insulin, lysozyme and myoglobin) on the surface of hyperbranched polymers, specifically, water-soluble oligosaccharide derivatives of polyethyleneimine, which are used as a stationery phase in capillary electrochromatography (CEC). Various protein molecules are known to be markers of different diseases. Detection and quantification of these proteins are of the highest importance for a successful disease diagnostics. However, irreversible adsorption of proteins onto the walls of the silica-fused capillary during the separation process may distort the results of the analysis. A method of ellipsometry is proposed to solve the problem. In the research we suggest to carry out the ellipsometric measurement mode in situ on the surface of a silicon wafer coated with a thin film of hyperbranched polyethyleneimine modified with maltose. According to the data of ellipsometric investigation, hyperbranched polymers are revealed to prevent protein adsorption during their electrophoretic separation. Key words : hyperbranched polyethyleneimine, ellipsometry, protein adsorption, capillary electrochromatography, PLOT-column. (Russian) DOI: http://dx.doi.org/10.15826/analitika.2014.18.1.009 V. Potolitsyna, L. Kartsova, E. Bessonova Saint-Petersburg State University (SPbU), St. Petersburg, Russian Federation
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