Abstract
Раннє виявлення та групова ідентифікація штамів Mycobacterium tuberculosis за допомогою SNP-аналізу зі шпилькоподібними праймерами
Highlights
single-nucleotide polymorphism (SNP)-àíàë3ç íà îñíîâ3 ïîë3ìåðàçíî¿ ëàíöþãîâî¿ ðåàêö3¿ (ÏËÐ) ç3 øïèëüêîïîä3áíèìè ïðàéìåðàìè äî ãðóïîñïåöèô3÷íîãî SNP katG463 òà ñòàòèñòè÷íèé àíàë3ç êë3í3êî-åï3äåì3îëîã3÷íèõ ïîêàçíèê3â çàñòîñîâàíî äëÿ äîñë3äæåííÿ êë3í3÷íèõ çðàçê3â ìîêðîòèííÿ õâîðèõ íà òóáåðêóëüîç ëåãåíü ì
Based on the method of single-nucleotide polymorphism (SNP) determination with hairpin primers to perform a differential identification in clinical material of M. tuberculosis (Mtb) strains, which belong to the 1st or 2/3 principal genotypic groups (PGG), with the aim of shortening the terms of tuberculosis diagnosing and early detection of most clinically and epidemiologically-significant strains
PCR with the SNP-specific hairpin primers to group-specific SNP katG463, and statistical analysis of clinical/epidemiological categories of the patients were used for study of sputum clinical samples from patients with pulmonary tuberculosis living in Kyiv
Summary
SNP (singlenucleotide polymorphism)-àíàë3ç íà îñíîâ ïîë3ìåðàçíî¿ ëàíöþãîâî¿ ðåàêö3¿ (ÏËÐ) ç3 øïèëüêîïîä3áíèìè ïðàéìåðàìè äî ãðóïîñïåöèô3÷íîãî SNP katG463 òà ñòàòèñòè÷íèé àíàë3ç êë3í3êî-åï3äåì3îëîã3÷íèõ ïîêàçíèê3â çàñòîñîâàíî äëÿ äîñë3äæåííÿ êë3í3÷íèõ çðàçê3â ìîêðîòèííÿ õâîðèõ íà òóáåðêóëüîç ëåãåíü ì. Çà äîïîìîãîþ ÏËÐ-ñèñòåìè äèôåðåíö3éíîãî ãðóïîñïåöèô3÷íîãî âèÿâëåííÿ ÌÁÒ ç âèêîðèñòàííÿì øïèëüêîïîä3áíèõ ïðàéìåð3â äî SNP katG463 M. tuberculosis äåòåêòîâàíî â 47,8 % êë3í3÷íèõ çðàçê3â, ç ÿêèõ 57,6 % ñòàíîâëÿòü øòàìè ÏÃÃ-1 3 42,4 % – ÏÃÃ-2/3. SNP-àíàë3ç ç3 øïèëüêîïîä3áíèìè SNP-ñïåöèô3÷íèìè ïðàéìåðàìè äî ëîêóñó katG463 ãðóïîâî¿ ïðèíàëåæíîñò øòàì3â ÌÁÒ äîçâîëÿo åôåêòèâíî âèÿâëÿòè ó êë3í3÷íîìó ìàòåð3àë åï3äåì3îëîã3÷íî çíà÷óù øòàìè M. tuberculosis ÏÃÃ-1. Íà îñíîâ ìåòîäó âèçíà÷åííÿ SNP çà äîïîìîãîþ øïèëüêîïîä3áíèõ ïðàéìåð3â ìè ðîçðîáèëè ïðîñòó ìåòîäèêó äèôåðåíö3éíîãî âèÿâëåííÿ ó êë3í3÷íîìó ìàòåð3àë øòàì3â ÌÁÒ, ùî íàëåæàòü äî 1-¿ àáî 2/3 ïðèíöèïîâèõ ãåíîòèïîâèõ ãðóï M. tuberculosis, äëÿ ïðèñêîðåííÿ ä3àãíîñòèêè òóáåðêóëüîçó 3 ïîøóêó â êë3í3÷íîìó ìàòåð3àë êë3í3÷íî 3 åï3äåì3îëîã3÷íî çíà÷óùèõ øòàì3â. Òàáëèöÿ 1 Õàðàêòåðèñòèêà êë3í3÷íèõ çðàçê3â çà ñîö3àëüíî-äåìîãðàô3÷íèìè òà êë3í3÷íèìè äàíèìè ïàö3oíò3â
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