ИЗУЧЕНИЕ ПРОТИВОВОСПАЛИТЕЛЬНОЙ АКТИВНОСТИ ВОДНОГО ЭКСТРАКТА ЛИСТЬЕВ CIRSIUM HETEROPHYLLUM (L.) HILL

Abstract

The biological activity of extracts from Cirsium heterophyllum leaves is of interest. Traditional medicine and preliminary studies indicate that this raw material may have the potential for anti-inflammatory activity. The aim of this study was to evaluate the anti-inflammatory activity of an aqueous extract of Cirsium heterophyllum leaves. We studied the anti-inflammatory activity of aqueous extracts of Cirsium heterophyllum leaves harvested in Vasilyevka village, Perm district, Perm territory (kray), Russia. The extracts were standardized for the content of flavonoids in terms of cynaroside. To study the anti-inflammatory activity, we used carrageenan-induced paw edema model in outbred ICR (CD-1) mice and outbred Wistar rats. Diclofenac was used as a reference drug. The test substances were dissolved in a 2% starch solution and injected intraperitoneally to the animals. The paw volume was measured hydrometrically and photometrically. The study has shown that the aqueous extracts of Cirsium heterophyllum have a mild effect compared to diclofenac. In mice, the percentage of inhibition of edema at the 4th hour was 19% (p-value = 0.0310) and 20% (p-value = 0.0130) for photometry and hydrometry, respectively. In rats, the percentage of edema inhibition was 47% (p-value = 0.0300) at the 1st hour and 28.7% (p-value = 0.0170) at 3 hours for hydrometry; photometry did not confirm these results. The results suggest that the aqueous extract of Cirsium heterophyllum has a mild activity in comparison with diclofenac, which may be due to the mutual action of the extract substances when applied intraperitoneally, as well as the presence of polysaccharides in the aqueous extract. Further research is required to determine whether the anti-inflammatory activity of its aqueous extract might be better combined with wound healing effects when applied topically.