Abstract
Soil is a vital and valuable natural resource that sustains life on earth. Appropriate soil functioning depends on the balance of its structure and composition, as well as physical, chemical and biological properties. Often, this balance is disturbed under the influence of various abiotic, biotic and anthropogenic factors. Therefore, soil restoration is of great importance in order to prevent possible adverse effects on living systems and to preserve the environment for future generations. Various studies confirmed the effectiveness of introduction of rhizobacteria to improve soil fertility, increase growth and productivity of agricultural crops. Bacillus subtilis is one of the most common rhizobacteria used in agriculture. Many B. subtilis strains are capable of fixing atmospheric nitrogen, solubilizing phosphorus and potassium, thereby contributing to an increase in the amount of macroelements necessary for plant nutrition in soil. The aim of this work was to search for genes responsible for synthesis of phytase, nitrogenase, and alkaline phosphatase enzymes in strains of bacteria of Bacillus subtilis species by real-time PCR. To determine the presence of genes encoding the synthesis of the desired enzymes in Bacillus subtilis, an in-silico analysis of the annotated genomes of this bacterial species presented in the NCBI information database was carried out. Then the selection of primers for screening the target spots was made. According to the results of the study, 10 out of 19 isolated Bacillus subtilis strains contained all three required DNA regions responsible for synthesis of phytase, nitrogenase, and alkaline phosphatase enzymes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
