Біофізичні властивості шкіри щурів із метаболічним синдромом

Abstract

It is known that metabolic syndrome (MS) prolongs the healing time of the wound surface of various genesis. We hypothesized that this may be related to changes in the biophysical properties of the skin. The aim of the work was to investigate the biophysical properties of skin (content of moisture, collagen, fused gelatin in the skin, and skin thermal fusion temperature) in rats with MS. Materials and methods. Research was conducted on 40 white non-linear rats (20 – males, 20 – females). After birth, males and females were divided into 2 groups. The first group served as a control for the second groups, in which MS was simulated by administering monosodium glutamate at a dose of 4.0 mg/kg on the 2nd, 4th, 6th, 8th, and 10th postnatal days. At the age of 4 months, the animals were decapitated under ether anesthesia. Biochemical studies were conducted to confirm the development of MS. The percentage of moisture, collagen content, destructive changes in collagen fibers and resistance of skin collagen to thermal factors were determined in native skin. The results. 4-month-old rats injected with monosodium glutamate in the early neonatal period developed MS as evidenced by abdominal obesity, insulin resistance, and dyslipidemia. In comparison with animals of the control group, in the skin of male and female rats with MS, the moisture content decreased, and the collagen content did not change. At the same time, the percentage of melted gelatin doubled, which indicates the depth of destructive changes in collagen fibers. Thermal welding of skin samples of male and female rats with signs of MS occurred at rather low temperatures compared to rats of the control group. Conclusion. Changes in the biophysical properties of the skin, which are registered in rats with MS, may be the reason for the later closure of the wound surface.