베르베린이 마우스 전염증성 사이토카인의 생성에 미치는 영향

Abstract

본 연구에서는 berberine이 전염증성사이토카인의 생성에 미치는 효과를 관찰하기 위하여 TNF-<TEX>$\alpha$</TEX>, IL-<TEX>$1{\beta}$</TEX>, 그리고 IL-6생성을 정량하였다. 마우스 비장세포에 berberine을 작용시켰을 때 TNF-<TEX>$\alpha$</TEX>의 생성이 억제되었다. 또한 마우스 생체 내에서 LPS에 의한 TNF-<TEX>$\alpha$</TEX>의 상승이 berberine에 의해서 억제됨을 알 수 있었다. IL-<TEX>$1{\beta}$</TEX>의 생성에 있어서도 berberine을 고농도(3.0 <TEX>${\mu}g/ml$</TEX>)로 작용시켰을 때 억제되었고 LPS에 의한 IL-<TEX>$1{\beta}$</TEX>의 상승이 고농도의 berberine에 의해서 억제되었다. IL-6의 생성은 berberine에 의해서 억제되었으며 낮은 berberine 농도(0.3 <TEX>${\mu}g/ml$</TEX>)에서 LPS에 의한IL-6의 생성이 억제되었다. 따라서 이러한 결과들은 berberine이 TNF-<TEX>$\alpha$</TEX>, IL-<TEX>$1{\beta}$</TEX>, 그리고 IL-6와 같은 전염증성사이토카인의 생성을 하향 조절할 가능성을 시사한다 하겠다. Berberine has shown a number of beneficial effects, including anti-tumor, anti-inflammation, and vasodilatory effects. In this work we investigated the effects of berberine on the production of proinflammatory cytokines such as TNF-<TEX>$\alpha$</TEX>, IL-<TEX>$1{\beta}$</TEX>, and IL-6 in mice. The supernatants of cultured splenocytes exposed with berberine or berberine plus LPS were harvested to assay TNF-<TEX>$\alpha$</TEX>, IL-<TEX>$1{\beta}$</TEX>, and IL-6. The sera from the mice injected with berberine or berberine plus LPS were then isolated to assay these cytokines. The TNF-<TEX>$\alpha$</TEX> production in mice splenocyte cultures exposed to berberine was inhibited compared to the PBS control. The sera from LPS plus berberine injected mice showed lower levels of TNF-<TEX>$\alpha$</TEX> compared to those of LPS only injected mice. The IL-<TEX>$1{\beta}$</TEX> production in mice splenocyte cultures exposed to berberine was inhibited at a high dose (3.0 <TEX>${\mu}g/ml$</TEX>) compared to the PBS control. Also, the increase of IL-<TEX>$1{\beta}$</TEX> by LPS exposure in splenocyte cultures was inhibited by a high dose of berberine. The IL-6 in splenocyte culture supernatants showed lower levels after berberine compared to the PBS control. Also, production of IL-6 after LPS exposure in splenocyte cultures was inhibited by a low dose of berberine (0.3 <TEX>${\mu}g/ml$</TEX>). These findings suggest the probability that berberine down-regulates the production of proinflammatory cytokines such as TNF-<TEX>$\alpha$</TEX>, IL-<TEX>$1{\beta}$</TEX>, and IL-6.