Abstract
Gamma (γ)-oryzanol is a substance abundant in rice, which is widely cultivated in Asian countries. In this study, we evaluated the effect of γ-oryzanol treatment on proliferation and apoptosis of AGS human gastric carcinoma cells. AGS cells were treated with γ-oryzanol for 72 h in a dose dependent manner. Treatment of γ-oryzanol (50, 100, and 200 μg/mL) resulted in decreased AGS cell proliferation and increased number of cells in the sub-G1 population. Additionally, apoptotic cells were investigated by annexin V staining and mitochondrial membrane potential assays. Our results indicated that γ-oryzanol treatment increased the number of annexin V-positive cells and depolarized cells. This demonstrated that γ-oryzanol is effective for the induction of apoptosis in AGS cells. We next examined the expression of promising anticancer drug target molecules, including PTEN and HSP90. We found that treatment of γ-oryzanol induced the expression of PTEN in AGS cells. Under the same treatment conditions, γ-oryzanol reduced the expression of HSP90 in AGS cells. These results suggest that γ-oryzanol-induced apoptosis was accompanied by changes in regulation of PTEN and HSP90 in AGS cells. Taken together, γ-oryzanol could be used as a functional substance for the prevention of gastric cancer.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
