토착 담수미세조류 메탄올 추출물의 항산화 및 항암효과

Abstract

In this study, antioxidant and anticancer activities of methanolic extracts from newly isolated freshwater microalgae were investigated. First, four microalgal strains in Korea were identified as Mychonastes sp. 246, Mychonastes sp. 247, Mychonastes pushpae 248, and Mychonastes sp. 249 through 5.8S-ITS2 gene sequencing and their carotenoid contents were analyzed. Then, we accessed antioxidant, cytotoxic, and anticancer activities of microalgal methanolic extracts. We found that the genus Mychonastes contained 226~2,607 μg/g, 138~1,594 μg/g, and 126~339 μg/g of β-carotene, lutein, and zeaxanthin, respectively. All of the microalgal extracts showed increased antioxidant and anticancer activities in a dose-dependent manner. Antioxidant activities of the extracts were 31 ± 7%, 27 ± 8%, 23 ± 7%, and 17 ± 8%, respectively, when treated with 50 mg/mL. From the results of cytotoxicity test, we did not find any serious growth inhibition of human mesenchymal stem cells treated at 10 μg/mL to 200 μg/mL. In addition, the microalgal extracts could effectively inhibit growth of all tested A549, BxPC-3, HepG2, and SKOV-3 cancer cells ranged from 16% to 62% when treated with 200 μg/mL. These results indicate the methanolic extract of four strain of genus Mychonastes could be used as a potential natural material as pharmaceutical ingredients.