음양곽(淫羊藿) 발효 추출물이 면역활성에 미치는 영향

Abstract

ABSTRACT Objectives : This research aimed at studying the immuno modulating activity of Fermented Epimedii Herba (EHS).Method : The impacts on the cell viability, hydrogen peroxide and nitric oxide (NO) generation in cells, and cytokines such as tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, IL-1β, monocyte chemoattractant protein-1 (MCP-1) level have been measured by using Raw 264.7 cells with the specimen EHS as the fermented extract of Epimedii Herba with Saccharomyces cerevisiae STV89.Result : As a result of MTT assay to confirm the cytotoxicity of extrac ts from fermented Epimedii Herba, the toxicity was not excessively induced in Raw 264.7 cells when EHS were processed by concentration. EHS increased hydrogen peroxide generation in Raw 264.7 cells. EHS suppressed NO generation in Raw 264.7 cells while they significantly suppressed the increase of NO generati on induced by LPS in macrophage. EHS significantly decreased the generation amount of TNF-α and IL-6 induced by LPS in Raw 264.7 cells at 25 ㎍/mL or more.Conclusion : It appeared that the fermented extract of Epimedii Herba manuf actured from Epimedii Herba significantly has the immuno modulating acitivity as it did not excessively trigger cytotoxicity to Raw 264.7 cells, increased hydrogen peroxide generation in Raw 264.7 cell s, decreased NO generation in macrophage, and especially, suppressed both TNF-α and IL-6 generation in macrophage induced by LPS.Key words : Epimedii Herba, ferment, cytotoxicity, immuno modulating aciti vity, reactive oxygen species (ROS)