Abstract
Due to the spreading and increasing drug resistance of pathogens, the search for novel antibiotics is becoming ever more important. Plant-derived polyphenols are a vast and promising class of compounds with a potential to fight infectious diseases. Still, they are not routinely used in clinical practice. No reports on the in vivo studies of these compounds have been presented. The aim of our work was to compare the antimicrobial activity of resveratrol (stilbene), dihydroquercetin and dihydromyricetin (flavonols) extracted from the bark and wood of conifers against the dermatophytes Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. Using the radial diffusion assay, we established that dihydroquercetin, resveratrol and dihydromyricetin exhibit high activity against S. aureus even at the smallest possible concentrations of 0.22, 0.15, and 0.15 mM, respectively. In contrast, the highest achievable concentrations of these compounds in the solutions (21.5, 15.5 and 15.0 mM for dihydroquercetin, resveratrol and dihydromyricetin, respectively) have no effect on the growth of P. aeruginosa and C. albicans. These findings suggest that polyphenols derived from conifers could have a potential to be used as a medicine for topical application to treat bacterial infections of the skin caused by S. aureus.
Highlights
Even the highest possible concentrations of the polyphenols tested in our experiment (21.5, 15.5 and 15.0 mM, respectively) exhibited zero antimicrobial activity against the gram-negative bacterium P. aeruginosa and fungus C. albicans
Levomecol was active against S. aureus at the minimal concentration of 24.2 mM and against P. aeruginosa at 242.3 mM
Polyphenols have not been used as an alternative to antibiotics, which raises the question whether research should be continued to obtain a comprehensive description of their specific biological activity
Summary
Целью работы было провести сравнительное исследование антимикробной активности препаратов полифенолов ресвератрола (стильбен), дигидрокверцетина и дигидромерицетина (флавонолы), выделенных из коры и древесины хвойных пород, в отношении дерматофитов: Staphylococcus aureus, Pseudomonas aeruginosa и Candida albicans. В тесте на подавление роста газона индикаторных культур в условиях радиальной диффузии из лунки установлено, что все три соединения проявляют высокую активность в отношении S. aureus: концентрации 0,22 мМ для дигидрокверцетина, 0,15 мМ для ресвератрола и 0,15 мМ для дигидромерицетина превышают предел эффективности. Over 8,000 phenolic compounds of plant origin have been identified They are classified into simple phenols, derivatives of hydroxy cinnamyl and hydroxy benzyl alcohols, flavonoids, stilbenes, and lignans [1]. Flavonoids (chalcones, catechins, anthocyanins, leucoanthocyanins, flavones, flavanones, and flavonols) are found in abundance in bark, flowers, seeds and fruits Stilbenes, another group of polyphenols, have two benzene rings and are related to flavonoids. Other stilbenes, including pterostilbene, pinosylvin and rhaponticin, have exhibited a similar type of activity [2]
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