Abstract
A new microanalytical method was established, which uses a fluorescent spectro photometer for concentration determinations of monosodium 5-nitro-(2-p-carboxystyryl) furan (abbreviated NFS-Na). At the 0.002ppm level, recovery rates of NFS-Na in the muscle of carp and yellowtail were confirmed to be 69.8% and 63.1%. NFS-Na, which was included in carp muscle and in yellowtail muscle, was stable during 30 days of sample storage under the condition of freezing at -20°C. The times to decrease the concentration of NFS-Na in the muscle to 0.002ppm were obtained: 8 hours for carp bathed in medicated (10ppm) 20°C water, 72 hours for carp orally administered (50mg/kg B.W.) in 20°C water, 48 hours for yellowtail orally administered (50mg/kg B.W.) in 25°C water.
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