Abstract
Plasmids are individual genetic elements that are capable of autonomous replication in a bacterial cell. These structures are actively used for genetic modification of various microorganisms. The artificial introduction of recombinant plasmids causes an additional metabolic load on the recipient bacterium, which can lead to the loss of the genetically engineered construct. Modification of nodule bacteria in order to obtain highly effective nitrogen-fixing strains is of great interest. However, there are not many data on the stability of the artificial vectors inheritance among recombinant rhizobia strains. In this work, we assessed the stable inheritance of the pJN105ParaBAD vector by nodule bacteria of three main genera: Rhizobium, Sinorhizobium (Ensifer), and Mesorhizobium. It was shown that the stability of the recombinant plasmids inheritance in the studied strains depended on their taxonomic position. The slowest loss of plasmids was observed in bacteria of the genus Ensifer. On the contrary, quick elimination of the vector was observed in bacteria of the genus Mesorhizobium.
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