ЭКСПЕРИМЕНТАЛЬНОЕ ИССЛЕДОВАНИЕ ДЕЙСТВИЯ АНТИБАКТЕРИАЛЬНЫХ ПРЕПАРАТОВ НА БИОПЛЕНКИ PSEUDOMONAS AERUGINOSA

Abstract

The article presents the results of stages of biofilm formation and sensitivity to antibacterial drugs of Pseudomonas aeruginosa bacteria. The technique for preparation of drugs for investigation in an optical and scanning electron microscope has been developed, which allows one to consistently study populations of Pseudomonas aeruginosa bacteria without disturbing natural architectonics. The culture of gram-negative bacteria Pseudomonas aeruginosa №17 in the logarithmic growth phase of growth was used in the work. A solution of canned bile of cattle and pigs, antibiotics of different groups and disinfectants were used as antibacterial preparations. To study the morphology of the P. aeruginosa population, the preparations were fixed in vapor of 25% (by DW) solution of glutaraldehyde. For staining, pairs of a 1% aqueous solution of osmic acid (OSO4) were used, dehydrated with propylene oxide vapor. The action of antibacterial drugs of various groups were studied in the population of P. aeruginosa. Studying the sensitivity of bacteria by diffusion to agar using standard commercial discs, it was found that P. aeruginosa is sensitive to the β-lactam antibiotic group, aminoglycosides, quinolones. Experimental data on the study of the effect of antibacterial drugs on processes of biofilm formation expand the boundaries of knowledge in the field of reseaches of the adaptive capabilities of ubiquitous bacteria for long persistence in both the warm-blooded organism and in environmental objects.