항산화활성 최적 조건으로 추출한 산겨릅 열수 추출물의 LPS로 유도된 RAW 264.7 세포에 대한 항염증 효과

Abstract

In this study, we investigated the anti-inflammatory effects of hot-water-extracts of Acer tegmentosum extracted under optimal conditions to maximize antioxidative activities with respect to lipopolysaccharide (LPS)-induced RAW 264.7 cells. Acer tegmentosum extract was produced based on the response surface methodology (RSM). The result from a previous study (Kim et al. 2021) was used for the current investigation (extraction temperature: 89.34°C, extraction time: 7.36 h and solvent to solid ratio: 184.09). The optimized Acer tegmentosum extract remarkably inhibited lipopolysaccharide (LPS)-inducible nitric oxide (NO) secretion to culture media on RAW 264.7 macrophages. The pre-treatment of Acer tegmentosum extract also significantly prevented the secretion of pro-inflammatory cytokines, such as interleukin 1 beta (IL-1β), interleukin 6 (IL-6), and tumor necrosis factor-alpha (TNF-α) from LPS-induced RAW 264.7 cells. Therefore, the pre-treatment of the optimized extract based on RSM for maximizing antioxidative activities of Acer tegmentosum effectively attenuated LPS-inducible inflammatory responses in the murine macrophage cell lines.