Abstract
Endotoxin shock in rats led to intravenous bolus of bacterial lipopolysaccharide (LPS) was accompanied by oxidative stress and increase of NO level in livers, lungs, kidneys, hearts of animals. The intravenous administration of bienzyme superoxide dismutase - chondroitin sulphate - catalase conjugate (SOD-CHS-CAT) showed its anti-endotoxemia action in rats at preventive (before initiation of injury) and therapeutic (after start of injury) application during cytokine phase of injury and influence on further stages of the damage. The injection of antioxidant SOD-CHS-CAT conjugate in a therapeutic regime did not demonstrate significant alterations of No level in livers, lungs, kidneys, hearts of rats (as compared to indices of the rat group with LpS action only) and displayed protective action on the kidney function by altered urea and creatinine in blood. The therapeutic effect of SOD-CHS-CAT was demonstrated by increase in organism viability. The effects of intravenous injection of SOD-CHS-CAT in a rat model of endotoxin shock showed a variety in the activity of this conjugate in addition to prevention of NO conversion in peroxynitrite upon interaction with O 2 superoxide radical. The results of accelerated normalization of arterial blood pressure and heart rate, a significant decrease in rat lethality because of using SOD-CHS-CAT conjugate indicate the importance of the determination of action mechanism of the cardiovascular injury models associated with other vasoactive agents besides NO. The further research of vascular damage pathogenesis and evaluation of its role in oxidative stress (time and place) are of paramount importance.
Highlights
Endotoxin shock in rats led to intravenous bolus of bacterial lipopolysaccharide (LPS) was accompanied by oxidative stress and increase of NO level in livers, lungs, kidneys, hearts of animals
The injection of antioxidant SOD-CHS-CAT conjugate in a therapeutic regime did not demonstrate significant alterations of NO level in livers, lungs, kidneys, hearts of rats and displayed protective action on the kidney function by altered urea and creatinine in blood
The therapeutic effect of SOD-CHS-CAT was demonstrated by increase in organism viability
Summary
The injection of antioxidant SOD-CHS-CAT conjugate in a therapeutic regime did not demonstrate significant alterations of NO level in livers, lungs, kidneys, hearts of rats (as compared to indices of the rat group with LPS action only) and displayed protective action on the kidney function by altered urea and creatinine in blood. The effects of intravenous injection of SOD-CHS-CAT in a rat model of endotoxin shock showed a variety in the activity radical. Одним из её потенциальных эффективных средств стали антиоксидантные ферменты, совершенствование форм которых ускоряет развитие и повышает действенность антиоксидантного лечения [11, 12]. Такие эффекты были выявлены в ходе последовательного изучения биферментного конъюгата супероксиддисмутаза – хондроитинсульфат – каталаза (СОД-ХС-КАТ) [1, 3, 11, 12].
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