Abstract
Products of plant raw materials processing, in particular hyssop herb are becoming more and more popular nowadays. This plant raw material is used in traditional medicine in many countries all over of the world and is included as an officinal raw material in pharmacopoeias of several European countries: France, Portugal, Romania, Sweden and Germany. Hyssop hydrolat is used in perfumery, cosmetics and food industries, but it is not used in medicine yet, due to the lack of a complex research. This article presents the results of a research of the chemical composition, antibacterial and antioxidant activity of Hyssopus officinalis L. hydrolate. The main aim of the article is to study the chemical composition, antibacterial and antioxidant activity of hydrolate of Hyssopus officinalis L. Materials and methods. The object of the research is a hydrolate of hyssop obtained by the method of steam distillation from freshly harvested raw materials of hyssop (Hyssopus officinalis L.). The study of the chemical composition of the hydrolate was carried out by gas-liquid chromatography. The antibacterial activity of the hydrolate was assessed by the growth and luminescence characteristics of the marine luminescent bacteria Aliivibrio fischeri F1 and recombinant bioluminescent test-objects based on E. coli MG1655. Antioxidant activity was determined by the method of non-enzymatic initiation of lipid peroxidation in a model biological system of yolk lipoproteins. Results. The concentration of essential oil in the hyssop’s hydrolate was defined as 0.032%. It was found that the hydrolate contains isopinocamphone, pinocamphone; β-cariophillen; β-pinen; sabinen and 1.8-cineol. The dynamics of the accumulation of lipid peroxide products in the presence of hyssop’s hydrolate was significantly lower in comparison with the control by 39.47%, which indicates the ability of this hydrolate to suppress free radical oxidation processes, and thus exhibit antioxidant activity. Conclusion. As a result of the research, hydrolate of Hyssopus officinalis L. was found to exhibit antibacterial and antioxidant properties. Prospects for further research are the development of medicines and cosmetics based on hydrolate of Hyssopus officinalis L. with antibacterial and antioxidant properties.
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