Abstract
By the methods of FAMES analysis, multiplex PCR with group-specific primers, phase-contrast microscopy, electron microscopy, plasmids profile detection, SDS-PAGE, gradient ultracentrifugation and RAPD with M13 Bacillus sp. ONU15 and Bacillus sp. ONU29 strains active against Bradisia pilistriata Frey were identified and characterized. Fatty acids composition and multiplex reaction products make it possible to identify the strain as Bacillus thuringiensis. The strains are heterogenic according to whole cell proteins and plasmids composition and also as RAPD amplification products developed with M13 primer.
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