Abstract
Вивчення вуглеводної специфічності гемолітичного лектину блідої поганки (Amanita phalloides (Vaill. Fr.) Secr)
Highlights
According to the SDS-PAGE data, the polypeptide chain of phallolysin consists of one component with molecular weight of approximately IS kDa
The sensitivity of erythrocyte of different species to the hemolysis decreases in the following sequence: rabbit > rat > man
The obtained results indicate that phallolysin forms ion-permeable pores with a functional diameter smaller than 2.3 nm in the cell membranes of rabbit erythrocytes, t the same time the presence of polyethylene glycols does not influence the hemaglutinating activity that allows to study carbohydrate specificity of the death-cap mushroom lectin
Summary
Purification and some physical and chemical properties of a hemolytic lectin from death-cap mushroom According to the SDS-PAGE data, the polypeptide chain of phallolysin consists of one component with molecular weight of approximately IS kDa. The molecular weight of the lectin as determined by gel filtration on Toyopearl HW-55 is 35 kDa. Phallolysin is thermolabile, the heating during 30 min at 65 °С removes completely ifs hemolytic and hemaglutinating ac tivity. The sensitivity of erythrocyte of different species to the hemolysis decreases in the following sequence: rabbit > rat > man.
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