Abstract
Исследование взаимодействия бромистого этидия с самокомплементарным дезокситетрануклеотидом 5-d(A)СpGpT) в водном растворе методом 1Н-ЯМР спектроскопии
Highlights
Complex formation between dye ethidium bromide (3,8-diamino-6-phenyl-5-ethyl-phenanthridinium) and self-complementary deoxytetraribonucleoside triphosphate 5'd-(ApCpGpT) in aqueous salt solution has been studied by method of one-dimensional (500 MHz) and two-dimensional *H NMR spectroscopy (600 MHz)
Two-dimensional homonuclear ]H NMR spectroscopy was used for qualitative analysis of the nature of interactions of ethidium bromide with tetranucleotide
Concentration dependences of proton chemical shifts of the molecules have been measured at fixed temperature (T=298K)
Summary
Таким обра зом, при исследовании комплексообразования бромистого этидия с d(ACGT) следует учитывать возможности избирательного связывания красителя с определенными сайтами тетрамера как в дуплексной, так и в мономерной формах. Предварительно выполненные отнесения сигналов протонов тетрануклеотида и красителя позволили отождествить спектры смешанных растворов и выявить возможные связи ядер молекул бромистого этидия и тетрамера.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
