Выявление путей гибели клеток карциномы почки человека А498 под действием экстракта аврана лекарственного и флавоноидов зеленого чая с помощью флуоресцентных методов визуализации

Abstract

Using fluorescence methods for visualizing apoptosis and necrosis in human tumor cells in vitro, we compared the efficacy of antitumor effects on human kidney carcinoma cells A498 flavonoid-containing extracts: Gratiola officinalis and green tea at ultra low concentrations after 24 and 48 h. Fluorescent dyes (propidium iodide and acridine orange) were used in the “living and dead test”, which made it possible to identify the total number of dead cells by necrosis and apoptosis, as well as cells in which apoptosis started, and apoptotic bodies or pycnosis of the nucleus were detected. Avrana extract possessed the most pronounced cytostatic activity 48 h after exposure, reducing the number of cells in the field of view by 1.5 times in comparison with the control. The most pronounced cytotoxic activity was possessed by green tea extract at a concentration of 0.0288 mg / ml, and its severity increased 48 h after exposure. Avran’s extract showed cytotoxic activity at lower concentrations (0.0036 mg / ml).