Референтные интервалы массовых коэффициентов внутренних органов морских свинок

Abstract

In toxicological studies for the detection of target organs, organ mass coefficients and absolute organ weights of laboratory animals are one of the monitored parameters. The aim of this study is to determine reference intervals of organ mass coefficients in relation to body weight and brain weight, and absolute organ weights in guinea pigs. The indirect method of obtaining reference values was used to determine reference intervals, and data from 40 male and 61 female outbred guinea pigs obtained during animal health monitoring from 2021 to 2023 were included in the analysis. The initial step in obtaining reference intervals is to determine the presence of statistical outliers and their exclusion, for each parameter evaluated separately. Reference intervals were established based on Clinical and Laboratory Standards Institute recommendations for mass coefficient and absolute values of heart, lung, thymus, liver, spleen, kidney, adrenal glands, brain, testes, and ovaries. Due to the small number of reference values (less than 120), a robust method of calculating reference intervals with confidence intervals was used. To improve the prognostic significance of the obtained reference intervals, it is necessary to increase the size of the analysed sample of reference values (more than 120). From the perspective of bioethical norms, a retrospective approach to obtaining additional reference values should be used to achieve this goal.