Abstract
The aim of this study was to investigate functional food material for the antioxidant and biological activities of untreated and steam-treated Corni fructus extracts in extraction solvents (through hot-water extraction, 50% ethanol extraction, and 50% methanol extraction). The yield of UCH (hot-water extract of untreated Corni fructus) was 47.45% and it was higher than those of extracts (13.20-27.18%) obtained by the other extraction methods. The total phenolic and flavonoid contents were 12.23 g/100 g (SCE, 50% ethanol extract of steam-treated Corni fructus) and 5.08 g/100 g (SCE), respectively, and the total sugar content was 71.32 g/100 g (SCH, hot-water extract of steam-treated Corni fructus). The main organic acid components of the extracts were oxalic acid, citric acid, malic acid, formic acid, and gallic acid. The DPPH and ABTS radical scavenging activities of SCE at 1,000 μg/mL were 72.37% and 43.15%, respectively. The ferric-reducing antioxidant power of SCE at 1,000 μg/mL was 689.49 μM. The extracts were investigated for their function in L-132, RAW 264.7, HeLa, and MCF-7 cell lines. The SCE performed better than the other extracts in terms of its protective effects against oxidative stress in L-132 cells and increased the production of NO. Further the SCE showed antitumor activities against HeLa and MCF-7 cancer cells. Therefore, the SCE extracts is a good functional food material for the prevention of woman disease. Therefore, in our study, the SCE extracts is good functional food material for the prevention of oxidant, immunological, and tumor related disease.
Highlights
The aim of this study was to investigate functional food material for the antioxidant and biological activities of untreated and steam-treated Corni fructus extracts in extraction solvents
산수유나무(Cornus 는 officinalis) 층층나무과에 속하는 낙엽활목이며 오래전부터 가을에 성숙한 산수유나무의 붉 은 열매인 산수유(Corni 를 fructus) 따서 씨를 뽑아내고 햇볕 에 말린 것을 한약재로 많이 사용하고 있다[8,9]. 독특한 향과 강한 신맛 및 약간의 단맛을 내고 성질이 따뜻하여 간과 신장의 기운을 북돋아주고 항암효과, 항균효과, 이뇨 작용 및 혈압강하작용 등이 있으며, 단백질의 소화를 돕는 작용 등이 있다고 한방자료에 기록되어 있다[10]. 산수유의 성분으로는 malic acid, tartaric acid, gallic acid, ursolic acid 등 유기산과 morroniside, loganin, sweroside, methylmorroniside 등 iridoid 배당체[11] 및 tellimagrandin 1, tellinagrandin 2, isoterchebin(Cornus-tannin 1), gemin D, Cornusiin A, B, 와C 1,7-di-O-galloyl-D-sedoheptullose, Cornusiin D, E, 와F 같은 류 가 tannin [12] 있다. 산수유에 대한 연구로는 항산화 활성 [13,14], 항균작용[10], 항암효과 와 [15] 유용성분에 대한 연 구 가 [16] 있으나, 산수유의 강한 신맛으로 인해 이를 활용한 식품 산업적 활용은 제한적이다
UCH, hot-water extract of untreated Corni fructus; SCH, hot-water extract of steam-treated Corni fructus; UCE, 50% ethanol extract of untreated Corni fructus; SCE, 50% ethanol extract of steam-treated Corni fructus; UCM, 50% methanol extract of untreated Corni fructus; SCM, 50% methanol extract of
Summary
The aim of this study was to investigate functional food material for the antioxidant and biological activities of untreated and steam-treated Corni fructus extracts in extraction solvents (through hot-water extraction, 50% ethanol extraction, and 50% methanol extraction). The SCE extracts is a good functional food material for the prevention of woman disease. In our study, the SCE extracts is good functional food material for the prevention of oxidant, immunological, and tumor related disease. Key : words Corni fructus extract, steam-treat, antioxidant, biological activity. 이러한 단점을 보완하기 위해 직 접적 열처리가 아닌 간접적 열처리인 증기를 이용하여 유용 성분의 용출을 증진시킬 수 있는 증숙 공정이 이용되고 있다[17,18]. 따라서 본 연구에서는 산수유의 유용성분의 용출을 향상 시키고 산수유의 강한 신맛을 감소시키기 위해 증숙 공정을 전처리로 적용하였으며, 추출용매에 따른 산수유 추출물의 항산화, 면역활성 및 항암 효과를 비교 분석하여, 기능성 식품소재로의 활용 증대 가능성을 조사하였다. 이용하여 작성한 표준곡선으로 정량하였으며 표준물질은 , 로부터 구입하여 사용하였다 Sigma-Aldrich Co
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