Abstract
We developed a flow distributor for high-performance chromatographic processes during the purification of biopharmaceuticals. Twelve distributor candidates are provided, including four types of distributors with different-shaped holes, and three sub-types with different number of holes. We evaluated the flow distributor candidates by spreading bromophenol blue dye on the membrane , and selected the most suitable distributor Arc 45 deg. type. Using 10 ml columns with three different length-to-diamter (L/D) ratios (4.33, 0.92, and 0.34) of column, we measured peak asymmetry (As) values. The 10 ml column with 0.92 L/D ratio showed best As value. We also carried out flow experiments on the three sub-types of Arc 45 deg. distributors on the 0.92 L/D ratio 50 ml column and Arc 45 deg. 1:8 distributor showed best performance. The As values of this optimal flow distributor are 1.2 or less. In summary, this study provides optimal distributor values for chromatographic purification of biopharmaceuticals.
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