가미청기산(加味淸肌散)이 항산화와 염증성 사이토카인에 미치는 영향

Abstract

Objectives : Gami-Chunggisan extract (GCE) is one of the oriental traditional medicine. We investigated the antioxidant effect and reduction of pro-inflammatory cytokine as a functional ingredient for cosmetic products from the GCE. Methods : GCE was prepared by extracting with 80% ethanol. We analyzed total polyphenol and antioxidant activities. To evaluate antioxidant activity, we measured 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay. Also we measured the production of reactive oxygen species (ROS) and nitric oxide (NO) on Raw264.7 cells. We researched reduction of anti-inflammatory cytokines from concentration of GCE on Raw264.7 cells. Results : Total polyphenol quantity of GCE was included 46.6 mg/g. The GCE showed ABTS free radical scavenging ability with more than 89% at <TEX>$1000{\mu}g/m{\ell}$</TEX>. In addition the DPPH free radical scavenging ability from the GCE was activated over 93% at <TEX>$1000{\mu}g/m{\ell}$</TEX>. Production of the ROS was decreased by approximately 26%, upon the GCE treatment at concentration of <TEX>$100{\mu}g/m{\ell}$</TEX>. The GCE at <TEX>$100{\mu}g/m{\ell}$</TEX> concentration showed inhibitory effect on NO production by 38%. Production of IL-<TEX>$1{\beta}$</TEX> and IL-6 were decreased by approximately 56% and 36%, respectively upon GCE treatment at <TEX>$100{\mu}g/m{\ell}$</TEX>. Also, production of TNF-<TEX>${\alpha}$</TEX> was decreased by approximately 79% at <TEX>$100{\mu}g/m{\ell}$</TEX>. Moreover, the GCE showed inhibitory effects on the expression of the IL-<TEX>$1{\beta}$</TEX>, IL-6 and TNF-<TEX>${\alpha}$</TEX> genes in LPS-induced Raw 264.7 cells. Conclusions : From the results above, we conclude that the GCE indicated significant antioxidant effects and induced reduction of pro-inflammatory cytokine.