Abstract
One of the tasks in the research of the gene pool of fruit crops is a systematic phenotypic assessment of samples in collections and the study of their genetic diversity. To date, traditional methods of varietal study and DNA marking are used for this purpose, which make it possible to identify donors and sources for breeding and promising varieties for industrial cultivation. The aim of the research was a comprehensive phenotypic and genotypic evaluation of varieties and hybrids of sweet cherries of domestic breeding. A long-term phenotypic assessment, carried out against the background of unstable and stressful conditions of the growing year, made it possible to identify more plastic varieties of sweet cherries Kavkazskaya uluchshennaya, Kavkazskaya, Podarok leta, combining the signs of winter hardiness, drought resistance, resistance to coccomycosis, and productivity. Simultaneous assessment of sweet cherry varieties of local breeding based on the analysis of polymorphism of microsatellite loci made it possible to establish genetic characteristics and perform DNA certification. To analyze the polymorphism of the studied genotypes, we used 7 SSR loci that were first identified and mapped in the sweet cherry genome (Alaya, Madonna, Kavkazskaya, Kavkazskaya uluchshennaya, Krasna devitsa, Mak, Podarok leta, Yasno solnyshko, Luchezarnaya, Yuzhnaya). Analysis of the obtained DNA fingerprints made it possible to establish that all the studied varieties of sweet cherries have a unique allelic set. For DNA certification of cherry varieties, the most polymorphic SSR markers are recommended: EMPa018, EMPaS12, EMPa017, EMPa004, UCDCH17, UCDCH12 and UCDCH31. Sweet cherry varieties Madonna (early), Podarok leta (middle) and Alaya (late) have been identified as sources of a complex of adaptive and productive traits recommended for further breeding work, as well as varieties that are for laying new cherry plantations in the south of the country.
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