Abstract

환경시료에서 많이 분리되는 장내바이러스 중 폴리오바이러스, 콕사키바이러스, 에코바이러스를 이용하여 정수처리 공정별 제거율을 조사하였다. 양성 시료로 사용된 바이러스들의 회수율은 72-108%의 범위로 나타났다. 이들 3종 바이러스들을 대상으로 정수처리공정별 제거효율을 조사한 결과 약 99%의 바이러스가 침전단계에서 제거되어졌고, 후오존과 BAC 여과과정을 거치면서 100% 제거되어짐을 확인할 수 있었다. 6개의 세포주를 사용하여 바이러스별 감수성을 조사한 결과 폴리오바이러스는 BGMK 세포주, 콕사키바이러스는 Vero 및 BGMK 세포주가 다른 세포주에 비해 높은 감수성을 나타내었고, 반면 에코바이러스는 RD 세포주에서 가장 분리가 용이하였다. Poliovirus, coxsackievirus, and ecovirus isolates from environmental sources were compared with laboratory strains to determine their removal rate in the water treatment process. The recovery efficiencies of poliovirus, coxsackievirus and echovirus using positive strains ranged from <TEX>$72{\sim}108%$</TEX>. Regarding virus removal efficiency in water treatment of pilot-plants, about 99% of all of the viruses were removed in the sedimentation step and 100% of viruses were clearly removed through post-ozonation and BAC filteration. Using six cell lines tested viral sensitivity in cell line and showed different sensitivity as viruses. Polioviruses showed higher sensitivity in the BGMK cell line than other cells, and coxsackieviruses were detected in higher level in the BGMK and Vero cell lines. On the other hand, the RD cell line was useful in the isolation of ecoviruses.

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