ЭФФЕКТИВНОСТЬ ПРЕПАРАТА МЕЛАФЕН НА РАЗЛИЧНЫХ ЭТАПАХ КЛОНАЛЬНОГО МИКРОРАЗМНОЖЕНИЯ

Abstract

Application of the drug Melafen was studied at the stages of introduction, proliferation, micro-propagation, adaptation to non-sterile conditions and open ground conditions of grapevine varie-ties of various origins and directions of use. It was found that at the introduction stage, in or-der to improve the survival and regeneration of meristems, the growth regulator Melafen in a concentration of 10-7% should be added to the nutrient medium. Different varietal responses to the use of the drug was noted. The use of Mela-fen at the stage of introducing meristems into culture has a positive effect on the passage of the next stage - micropropagation (proliferation). The positive effect of the drug Melafen was re-vealed in the microcutting of plants of the Tsim-ladar grapevine variety. A clear improvement in all qualitative indicators was noted at concentra-tions of 10-7, 10-9, 10-11%. The best results were obtained at a concentration of 10-7. The variety-specificity of the drug's action to improve the characteristics of plants was noted. The "afteref-fect" of Melafen was found, which is expressed in an improvement in the condition of plants during subculture without Melafen. The length of the rhizogenic zone increased by 1.4–2.5 times, the height of plants by 1.3 times, the growth rate increased, and therefore, there is an acceleration of micro–propagation and, in gen-eral, an increase in the effectiveness of clonal micro-propagation. Improving the qualitative characteristics of plants contributes to the adap-tation process (higher yield of adapted plants) with good plant condition.