Папилломавирусная инфекция эякулята, ассоциированная с мужским фактором бесплодия

Abstract

Introduction Infertility is the complex and urgent problem in reproductology. Now HPV involvement in male infertility is considered proven. Objectives of the study: To study the etiological structure of human papillomavirus infection (HPV) in ejaculate in men with infertility, as well as to assess the effect of HPV on the parameters of sperm (motility, quantity, morphology). Materials and methods The analysis of the results of the examination of 71 patients aged 22 to 44 years with a diagnosis of infertility, combined with PVI, with the absence of other risk factors. The assessment of the fertilizing ability of the ejaculate was carried out in accordance with the data and parameters recommended by the WHO (2010) in the clinical diagnostic laboratory. To identify the type of virus used amplification method of DNA - diagnostics (polymerase chain reaction - PCR). The material for the study was ejaculate. Results The analysis of the ejaculate: asthenozoospermia was detected in 56% cases, asthenoteratozoospermia - 21%, oligoasthenoterozoospermia - 16%, oligoastenozoospermia - 6%. The most frequent HPV types were 16 (60,7%), 18 (54,9%), 33 (49,3%). The most frequent HPV types combinations were 16 with 18 (33,8%), 16 with 33 (35,2%), 18 with 33 (28,2%). The number of virus types has a statistically significant weak correlation with sperm motility (r=-0.267; p=0.0244). Combinations of types 6 and 11, 11 and 16, 16 and 33, 31 and 33 of HPV types more often than others worsened several sperm parameters rather than one. The combination of 6 and 11 HPV types significantly decreases progressive (6.20+/-4.18% and 10.52+/-5.66, p=0.026) and total (11.10+/-5.95% and 17.90+/-6.92%, p=0.0056) motility compared with other combinations of HPV types. The combination of 31 and 33 HPV types was characterized by a large decrease in the total sperm count (12.78 +/- 8.81 million and 21.82+/-9.92 million, p=0.011) and the count of sperm in 1 ml (29.11+/-21.54 million and 53, 35+/-22.13 ppm, p=0.0052) compared with other combinations of HPV types. Conclusions According to the results of the study, patients with idiomatic infertility, combined with PVI, have different variants of pathozoospermia.