Abstract
Recently, antigen-specific assays for detecting anti-platelet alloantibodies have been available: immunobead assay, modified antigen capture ELISA (MACE) and monoclonal antibody-specific immobilization of platelet antigens (MAIPA). These assays are based on essentially the same principle. In this study, using anti-HPA-1a and anti-HPA-4a antibody, we have compared the sensitivity of MACE using avidin-biotin-complex method (ABC-MACE), MACE, MAIPA, and flowcytometry (FCM) as a conventional method. The sensitivity of MACE for detecting anti-HPA-1a antibody was twofold better than that of MAIPA, while the sensitivity of MAIPA for detecting anti-HPA-4a antibody was twofold better than that of MACE. Using ABC-method, the sensitivity of MACE for detecting anti-HPA-la and anti-HPA-4a increased 8-fold and 64-fold, respectively. In comparison with FCM, ABCMACE showed almost the same sensitivity for detecting anti HPA-4a antibody, and showed better sensitivity for detecting anti HPA-1a antibody. These data demonstrate that ABC-MACE is much more sensitive than MACE or MAIPA for detecting anti-platelet alloantibodies, and that the sensitivity of ABC-MACE is comparable to that of FCM.
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