Abstract
Non-invasive methods for detecting plant stress conditions are a method for registering chlorophyll fluorescence, including measurement of induction and spectral analysis of fluorescence, and a method for spectral analysis of radiation reflected from leaves. Existing portable devices allow you to measure only one of these parameters. (Research purpose) The research purpose is in developing a combined portable device for detecting plant stress conditions by recording the reflective characteristics of leaves, the spectrum, and the induction of chlorophyll fluorescence. (Materials and methods) Authors developed a laboratory sample of the device for determining the stress states of plants and tested it on plants of garden basil (Ocimum basilicum) lemon varieties, while some plants were exposed to a stress factor in the form of ultraviolet C-radiation. Authors analyzed the fluorescence spectrum of chlorophyll when excited by various radiation sources and the back reflection spectrum when irradiated with a halogen lamp. (Results and discussion) The influence of the stress factor is manifested in a change in the shape of the fluorescence spectrum, namely, in a decrease in the intensity of fluorescence in the long-wave part of the spectrum. the reflectivity in the green and far red regions of the spectrum also decreases, and the steepness of the graph decreases when moving from the red to the far red region. (Conclusions) To register the fluorescence spectrum of chlorophyll, the best option for excitation is the use of lasers with wavelengths in the range of 405÷470 nanometers. It is possible to combine the functions of spectral analysis of chlorophyll fluorescence and radiation reflected from leaves in one device, which can improve the accuracy of determining the stress states of plants.
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