Abstract
New strains of Sinorhizobium meliloti were isolated from nodules of wild hop alfalfa growing in different districts of the Moscow region. Strain LX1 was isolated from the nodules of wild hop alfalfa, on the basis of which the Mira variety was created. Strain LX6 was isolated from alfalfa living in conditions of very low fertility and moisture deficiency at the Ivankovo reservoir dam. Strains LX2 and LX5 — on the railway embankment, LX3 and LX4 — on a relatively fertile area of the meadow. In the field experiment, LX1 strains provided the highest efficiency of symbiosis with the hop-shaped alfalfa variety. The increase in productivity for dry matter reached 81%, for seeds 87%. The LX6 strain increased seed yield by 86%. The best results for the selection number VIK 32-28 were obtained by inoculation with strains LX4 and LX6. Productivity for dry matter increased by 26 and 21%, for seeds — by 112 and 150%. In the vegetation experiment, an accelerated method of selecting highly effective macro- and micro symbionts in non-sterile conditions was optimized. On the 35th day after the emergence of seedlings, the herbage capacity was visually determined. The most powerful were the plant-microbial systems "variety of the Mira + strains LX3, LX6, LX2 and LX1". The height of elongated shoots was 11.8 ± 1.5 … 15.3 ± 1.6 cm, bushiness of 3.7 ± 0.5 … 4.0 ± 0.6 stems, symbiosis efficiency of 18–102%. Positive correlations were revealed between productivity and plant height (r = 0.42 ± 0.40), bushiness (r = 0.57 ± 0.36), number of nodules (r = 0.62 ± 0.35).
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