다공성 물질에서 인체 시료 채취를 위한 접착제의 적용

Abstract

Many of the crime scene evidences are collected by transferring body fluids that are attached to the surface using a tool such as a sterile cotton swab or tape. The amount of body fluids transferred to the collection tool directly affects the possibility of DNA profiling. In this study, the recovery rate of body fluids and the detection rate of DNA profile were examined using sterile cotton swabs, rubber coating agents and urethane-based adhesives from porous materials. We prepared porous samples by spraying 100㎕ of diluted blood on their surface, each sample was collected using a sterile cotton swab, rubber coating agent, and urethane-based adhesive, and DNA recovery rates were compared. In the result, urethane-based adhesives showed the highest recovery rate of DNA with 68.6%. Rubber coating agent and sterile cotton swab showed 47.2% and 2.9% of recovery rate, respectively. Furthermore, we measured the FT-IR spectra of the rubber coating agent and the urethane-based adhesive and found that the peak of C=O group was larger in urethane-based adhesive. This result indicates that the polarity of urethane-based adhesive is greater than that of rubber coating agent and it has excellent blood wettability. As a collection tool, urethane-based adhesive had the disadvantage of taking a long drying time in the sampling process, however, it showed a high recovery rate when collecting body fluids from porous samples, and possibility for use in actual incident cases was confirmed.