Abstract
본 연구는 버섯재배에 이용되는 배지재료를 이용하여 상업용 PDA 고체배지를 대체할 수 있는 원균 증식용 천연고체배지 제조 가능성에 대해 조사하였다. 천연고체배지는 큰느타리버섯 재배에 이용되는 재료를 열수 추출하여 제조하였다. 포플러톱밥, 밀기울, 미강이 버섯의 균사생육에 좋은 특성을 보였다. 천연 고체배지 제조를 위한 포플러톱밥, 밀기울, 미강의 적정 혼합 비율은 50 : 20 : 30(v/v/v, PWR523)와 50 : 30 : 20(v/v/v, PWR532) 수준이었다. 열수수출물 함유 천연 고체배지인 PWR523 및 PWR532에 대한 버섯 균사생육은 PDA 배지에 접종된 것보다 빠른 특성을 나타내었다. 또한 열수추출물 함유 천연 고체배지에 의해 만들어진 접종원을 이용한 경우 버섯의 배양 및 수확에 있어서 상업용 배지를 이용한 것과 큰 차이를 보이지 않았다. Hot-water extracted natural media were made from raw materials for mycelial culture of Pleurotus eryngii. Poplar sawdust, wheat bran and rice bran were used as substrates for hot water extraction. The mixed substrates of poplar sawdust, wheat bran, and rice bran with 50 : 20 : 30 (v/v/v, PWR523) and 50 : 30 : 20 (v/v/v, PWR532) were optimal for mycelial growth of P. eryngii, respectively. The hot-water extracted natural media from PWR523 and PWR532 showed a rapid mycelial growth and spawn running compared to PDA. There was no significant difference in mushroom yield when the mycelium grown on the hot-water extracted natural media was used as the inoculum source for producing fruit body.
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