Abstract
Naphthoquinones play an important role among natural substances and their synthetic derivatives, as they have a wide range of biological activity. Amino acid derivatives of 1,4-naphthoquinone have prospects for practical use in medicine and pharmacology. To clarify the possible mechanism of action of amino acid derivatives of 1,4-naphthoquinone, the content of TBA-positive products in embryonic cells of the loach Misgurnus fossilis L. during embryogenesis was investigated. Embryos of the freshwater fish loach Misgurnus fossilis L. are a convenient and adequate system for elucidating the mechanisms of action of physical and chemical factors. It has been established that the glutamine derivative of 1,4-naphthoquinone has the most pronounced biological effects and caused significant changes in TBK-positive products during embryogenesis relative to the control at stages 2 and 256 blastomeres (60 and 270 minutes of embryo development) by 47 % and 43 %, respectively (p≥0.99). At stages 16 and 64 blastomeres, there is a tendency to increase the content of MDA under the action of the glutamine derivative of 1,4-naphthoquinone at a concentration of 10-5 M compared to the control. Also, at the 10th division stage under the influence of the glutamine derivative of 1,4-naphthoquinone, the MDA content decreases by 2 % compared to the control sample, possibly due to the activation of enzymatic systems of antioxidant protection. Increasing the intensity of lipid peroxidation processes stimulates the activation of antioxidant system enzymes. Therefore, to analyze the prooxidant-antioxidant state of loach embryos, it is necessary to study the activity of enzymes that provide antioxidant protection. The obtained results allow us to suggest that under the action of the derivative of 1,4-naphthoquinone, activation of lipid peroxidation processes occurs in embryonic cells of the loach Misgurnus fossilis L. during embryogenesis.
Published Version
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