Abstract
Fire blight is a contagious disease affecting a wide range of host species within the family Rosaceae. The causal pathogen is Erwinia amylovora that belongs to the family Enterobacteriaceae. Many virulence determinants of E. amylovora have been characterized, including the Type III secretion system (T3SS), exopolysaccharides (EPS) amylovoran and levan. Amylovoran is a heteropolysaccharide with pentasaccharide repeating units containing four galactose and one glucuronic acid residues. Amylovoran is a pathogenicity factor in Erwinia amylovora, essential for the development of disease symptoms. It has been suggested to play a major role in disturbance and obstruction of the plant vascular system. Amylovoran and levan are required for biofilm formation by E.amylovora. In this study the following strains were used: Erwinia amylovora E2, D4. We demonstrate the influence of plant phenolic compounds on the production of E. amylovora EPS amylovoran and levan and catechol 2.3-dioxygenase activity. We showed that amylovoran production in presence in cultivation medium of cinnamic, vanillic and ferulic acids increased significantly (52.7%, 54.31% and 29.4% respectively). The presence in cultivation medium of caffeic acid did not influence amylovoran synthesis. Also we investigated that catechol 2.3-dioxygenase activity and amylovoran production was inhibited by salicylic acid (51% and 60% respectively). Unfortunately, we couldn’t demonstrate the influence of phenolic acids on levan production. The results indicate that plant phenolic compounds inhibit or increase amylovoran biosynthesis, however, it deserve further research.
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