Золотые нанозвезды как фотоагент при антимикробном воздействии инфракрасного (808 нм) лазерного излучения

Abstract

Gold nanostars with an average core diameter of 122,2 nm and a spike length of 114,6 nm were synthesized and characterized at a concentration of 5,36×1010 pcs/ml with an absorption maximum of 840 nm. Gold nanostars were coated with thiolated polyethylene glycol, its amount was about 8×104 molecules per 1 particle and about 4,4×1015 molecules/ml in the colloid. The zeta potential of gold nanostars coated with PEG-SH was −2.3 mV. The combined effect of gold nanostars and low-intensity infrared (808 nm) laser radiation on the bacteria Staphylococcus aureus 209 P and Escherichia coli 113-13 has been studied. Incubation of suspensions of microorganisms in the presence of nanoparticles without access to light did not lead to a significant reduction in the number of bacteria. Irradiation for 30 min of bacterial suspensions containing nanoparticles caused the death of 39% of the S. aureus population and 80% of the E. coli population. During the thermometry of the studied suspensions, it was found that the temperature increase is dose-dependent. The increase in temperature of the control samples that did not contain photothermal agents did not exceed 1 °C in both cases throughout the entire time of the experiment. For suspensions of bacteria (equally S. aureus and E. coli) incubated with gold nanostars during irradiation, an increase in temperature indicators, on average, by 4 °C was revealed. The more pronounced antibacterial activity of the combination of gold nanostars and infrared (808 nm) laser radiation against E. coli can also be explained by the greater sensitivity of gram-negative microorganisms to photothermal exposure.