Abstract
당뇨병의 정확한 진단을 위해 산업체에서는 2세대 포도당센서를 사용해왔다. 2세대 포도당센서의 전자전달 매개체는 철, 루테늄 및 오스뮴과 같은 VIII족 금속으로 이루어져 있으며 본 논문에서는 포도당 탈수소 효소(GDH) 와 감응하는 새로운 전자전달 매개체를 합성하였다. 전기화학적 포도당 센서용 polyvinylimidazole(PVI)-[루테늄(4,4"-diamino-2,2"-bipyridine)2Cl](PVI-[Ru(dam-bpy)2Cl]) 전자전달 매개체의 구조는 1H nuclear magnetic resonance, Fourier-transform infrared 및 ultraviolet-visible spectroscopy로 측정하였다. 또한, cyclic voltammetry와 multi-potential step을 사용하여 전기화학적 특성을 분석하였다. PVI-[Ru(dam-bpy)2Cl]은 GDH를 사용하여 전극을 제작하였고, 마지막으로, 광범위한 포도당 농도(1-40 mM)에서 전기화학적 선형성이 입증되었다. PVI-[Ru(dam-bpy)2Cl] 전자전달 매개체가 2세대 포도당 바이오센서로서 산업계에서 직접 사용될 수 있을 것으로 기대한다. Diabetes necessitates the use of reliable glucose sensors capable of preventing the onset of complications via accurate and timely diagnosis. Among such sensors is a second-generation glucose amperometric sensor that uses an electrochemical method and is based on a mediator that transfers electrons from the enzyme to the electrode. Group VIII metals, such as iron, ruthenium, and osmium, have conventionally been used, and are synthesized with both electron-withdrawing and -donating ligands. Among these mediators, hexaamineruthenium(III) transferred electrons generated by glucose via glucose oxidase (GOx) to the electrode, allowing glucose quantification. However, a measurement error of the GOx enzyme was recently discovered due to external oxygen interference; therefore, it is being replaced with the enzyme glucose dehydrogenase (GDH). Hexaamineruthenium(III) exhibited a low electron transfer efficiency due to electrons stolen during the reaction with the GDH enzyme. Thus, to solve this problem, herein we synthesized a polymer-based ruthenium mediator complex, viz. polyvinylimidazole-[ruthenium(4,4"-diamino-2,2"-bipyridine)2chloride] (PVI-[Ru(dam-bpy)2Cl]) for electrochemical glucose sensing. The structure of the new mediator was determined using 1H nuclear magnetic resonance, Fourier-transform infrared, and ultraviolet-visible spectroscopies. Furthermore, electrochemical properties were determined using cyclic voltammetry and a multi-potential step. PVI-[Ru(dam-bpy)2Cl] interacted favorably with GDH. Finally, electrochemical linearity was demonstrated with a wide range of glucose concentrations (1-40 mM). We anticipate that PVI-[Ru(dam-bpy)2Cl] can be used directly in the industry as a second-generation glucose biosensor.
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