가미계작지모탕(加味桂芍知母湯) 주정 추출물이 류마티스 관절염 인자에 미치는 영향

Abstract

ABSTRACT Objectives : The purpose of this study is to prove the effect of Gamikyejakjimo-tang (jiāweiguishaozhīm-tāng, GK) on rheumatoid arthritis.Methods : We checked viability and measured production of IL-1β, IL-6, IL-17, TNF-α in RAW 264.7 cell after treat by GK. Then we measured rheumatoid arthritis index score of DBA/1 mice with rheumatoid arthritis induced by CIA after GK oral administration, checked IL-1β, IL-6, IL-17, TNF-α and hs-CRP tests in serum. Also we were observed mRNA expression of IL-1β, IL-6, IL-17 and TNF-α in spleen by RT-PCR.Results : GK showed cell viability of 100% or higher in all concentration in RAW 264.7 cells. GK inhibited LPS-induced productions of rheumatoid arthritis mediators cytok ine in RAW 264.7cells. GK treated group showed improvement from rheumatoid arthritis at decreased the i ndex score. Also, GK treated group decreased level in serum of IL-1b, IL-6, IL-17, TNF-a and hs-CRP tests by 31%, 35%, 20%, 57% and 58% respectively. Finally, GK treated group showed decrease expression of IL-1β, IL-6, IL-17 and TNF-α mRNA in spleen by 46%, 51%, 25% and 42% respectively.Conclusions : In this study, in-vitro and in-vivo results observed rheumatoid arthritis factors cytokine of IL-1β, IL-6, IL-17 and TNF-α decrease in RAW 264.7 cells, serum, mRNA expression. Also, GK showed decrease of inflammation figure in hs-CRP tests depending on effect of r heumatoid arthritis. Thus, these results can used as a effective drug of GK for rheumatoid arthritis.Key words : Cytokine, hs-CRP, Gamikyejakjimo-tang, mRNA, Rheumatoid arthri tis, serum