Микробиом эндометрия у женщин с многократными неудачами экстракорпорального оплодотворения

Abstract

Objective. To assess endometrial microbiome in women with infertility and multiple failed in vitro fertilization (IVF) cycles. Patients and methods. The study included 42 women; group 1 consisted of 22 women aged 20 to 42 with infertility and repeated unsuccessful IVF cycles; in group 2 (control), there were 20 healthy women aged 20 to 42 years, planning pregnancy. Microbiome samples for the study were taken from 20 to 24 days of the menstrual cycle. Results. Higher relative concentrations of Lactobacillus iners, Lactobacillus acidophilus, Lactobacillus jensenii, Lactobacillus crispatus, Prevotella melaninogenica, Bacteroides vulgatus, Corynebacterium bouchesdurhonense, Bacteroides caccae, Bifidobacterium gallinarum, Bifidobacterium adolescentis were revealed in the group of healthy women without aggravating factors in obstetric and gynecological medical history. Higher relative concentrations of Methylobacterium aerolatum and Comamonas testosteroni were found in women in group 1. A distinctive feature of endometrial microbiota in women in this group was the presence of Streptococcus spp. and Gardnerella vaginalis in low concentrations. The average relative representation of the genus Lactobacillus was 34.4% in group 1 and 63.0% in group 2. In general, the composition of the uterine microbiome contained bacteria characteristic of oral and intestinal biotopes. Conclusion. Women with multiple failed IVF cycles have greater biodiversity than healthy women. The presence of high concentrations of Lactobacillus may be a marker of favorable reproductive outcomes. Key words: endometrial microbiome, failure, in vitro fertilization, Lactobacillus