Abstract
Выбор условий витрификации макропористых матриц, заселенных мезенхимальными стромальными клетками
Highlights
The composition of the solution of cryoprotectants and vitrification conditions of tissue engineered constructs based on macroporous matrices (MPM) and mesenchymal stromal cells (MSCs), preventing the development of crystallization and cracking of a glassy phase, were determined and the influence of the developed conditions on cell viability and MPM structure was studied
The use of tissue-engineering constructs (TECs) based on macroporous matrices (MPM) and multipotent mesenchymal stromal cells (MSCs) is a promising area for the rehabilitation of damaged tissues [4, 9], which is getting increasingly important in regenerative medicine
We previously showed that a solution containing 10% dimethyl sulfoxide (DMSO); 20% ethylene glycol (EG); 20% 1,2-PD; 0.5 M sucrose проблеми кріобіології і кріомедицини problems of cryobiology and cryomedicine том/volume 30, No/issue 1, 2020 неспособность мезенхимальных стромальных клеток (МСК) в составе макропористих матриц (МПМ) оценивали по двойному окрашиванию этидиум бромидом (ЭБ)/ флуоресцеин диацетатом (ФДА) [6]
Summary
The composition of the solution of cryoprotectants and vitrification conditions (saturation methods, removal of cryoprotectants, cooling-warming modes) of tissue engineered constructs based on macroporous matrices (MPM) and mesenchymal stromal cells (MSCs), preventing the development of crystallization and cracking of a glassy phase, were determined and the influence of the developed conditions on cell viability and MPM structure was studied. Successful implementation of the TEC potential requires the available effective methods for their long-term storage and establishing the banks of Надійшла 28.08.2018 Прийнята до друку 11.02.2020.
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